Questions to study.
1. Acetyl CoA: its structure, significance and pathway of its synthesis.
2. Citric acid cycle: partial reactions, regulation and biological significance.
3. Tissue respiration. Structure of mitochondrial respiratory chain. Functioning of the respiratory chain. Synthesis of endogenous water in mitochondria.
4. Formation of incomplete oxygen reduction products and their detoxification in the cell.
5. Complexes of mitochondrial respiratory chain and their relationship.
6. Inhibitors of tissue respiration: their classification and representatives.
7. Pathways of ATP generation. Oxidative and substrate-level phosphory-lation.
8. Oxidative phosphorylation. P. Mitchell's chemiosmotic hypothesis.
9. Mechanism of proton gradient formation on the inner mitochondrial membrane.
10. Components of energy of the proton gradient, and pathways of its use.
11. Structure of coupling factor (ATP-synthase) and its role in the synthesis of ATP.
12. Uncouplers of oxidative phosphorylation.
13. Evaluating the effectiveness of oxidative phosphorylation. P/O ratio.
14. Regulation of oxidative phosphorylation. Respiratory control.
TESTS TO ASSESS FORMATION OF THE FOLLOWING COMPETENCIES: GC-1, GPC-1, 7, 9, P-5, 12
Choose one correct answer
3.1. Indicate the reaction of substrate-level phosphorylation in the Krebs cycle:
A) malate dehydrogenase;
B) aconitase;
C) succinyl-CoA-synthase;
D) succinate dehydrogenase.
3.2. Indicate dehydrogenases of the Krebs cycle:
A) isocitrate dehydrogenase;
B) α-ketoglutarate dehydrogenase;
C) succinate dehydrogenase;
D) malate dehydrogenase;
E) all of the above.
3.3. Krebs cycle enzymes are located in:
A) cytosol;
B) microsomes;
C) mitochondria;
D) lysosomes;
E) peroxisomes.